Exploring Multiplexed Proteomics in Complex Applications with SpectroMine
HUPO Connect 2020 Seminar | October 21st, 2020
"Multiplexed Proteomics for Understanding Molecular Biology"
Mikhail Savitski, PhD (Team Leader & Head of Proteomics Facility - EMBL)
An application of multiplexed proteomics for investigating molecular biology will be presented. In particular, examples of how thermal proteome profiling can provide insights into the functional aspects of the proteome will be discussed. The seminar will highlight the benefit of using SpectroMine for these types of experiments.
"Characterizing Cellular Hierarchies Using Quantitative Single-Cell Proteomics"
Erwin Schoof, PhD (Assistant Professor - Technical University of Denmark)
A single-cell proteomics strategy was used to investigate the characteristics of individual cells in acute myeloid leukemia (AML). Taking advantage of the latest advancements in LC-MS instrumentation and data acquisition, the new TMTPro reagents, and SpectroMine 2.0, an unprecedented map of protein expression was built-in individual AML cells. Furthermore, a computational pipeline (SCeptre) was built that effectively processes single-cell proteomics data and permits the extraction of cell-specific proteins. Strong enrichment of stem cell-specific proteins was found in the LSC and progenitor compartments, and protein expression signatures were identified that clearly distinguish cell differentiation stages. This work lays a solid foundation for implementing global single-cell proteomics studies in proteomics labs across the world.